cbr_rachie
Club Member
yeh so i'm stuck at work til 5, and i finished my work and i've had enough of myspace.....soooooooooo what's up d-town? how's everyone been lately?
bored as hell at work, entertain me ppls!!!
- Thread starter cbr_rachie
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Captain Slow
Still waiting on our new HPLC column...it's been 3 and a half weeks and I still haven't gotten this thing to work correctly yet...so, yeah...I'm bored here at work, too. Can't do anything until that column shows up
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Captain Slow
High pressure liquid chromatography column...it's a metal tube filled with shit that separates out different shit in a solution at different times...basically
More in depth...
You have a mobile phase...ours is reversed phase so it is a combination of Acetonitrile and water. This mobile phase gets pumped, under high pressure (around 1000psi) through the LC system that includes an autosampler, a pump, a column, and a UV spectrometer.
The autosampler is a motorized needle and a tray that withdraws and injects a certain amount of a prepared standard or sample into the LC machine.
The pump does just that...pumps the mobile phase with or without the sample/standard through the system
The column is a tube filled with different compounds that slow down the progress of the compounds in the standard or sample. Think of it as a tube filled with marbles. If you pour water through it, the water will eventually drain to the bottom. If you pour something more viscous, like oil, it will take a little longer because the substance is thicker than the water. If you try to pour something really viscous, like honey, it will take a lot longer because the substance is thicker than the oil. Molecules work the same way...some molecules are more attracted to the solid phase (the compound bonded into the inside of the column) than others. The ones that are less attracted to the column solid phase will work their way through the column faster than the compounds that are more attracted to the solid phase.
The UV Spectrometer reads when the compound makes it through the column. It detects the presence of the specific molecule by hitting it with a specific wavelength of light. The light interacts with the molecule, and the detector in the Spectrometer records this interaction.
The signal from the detector is then analyzed and sent to a recording software or to an integrator that prints out a chart.
The signals are represented as peaks. The location of the peak is the retention time of the specific molecule, and shows WHAT the molecule is. The area under the peak shows HOW MUCH of that molecule there is in the sample/standard.
The column we got is not separating the compounds we are looking at correctly, so we are waiting for another column that will (hopefully) separate them well enough to get some useable data so we can start analyzing the various polymers I have been making for the last 3 months
More in depth...
You have a mobile phase...ours is reversed phase so it is a combination of Acetonitrile and water. This mobile phase gets pumped, under high pressure (around 1000psi) through the LC system that includes an autosampler, a pump, a column, and a UV spectrometer.
The autosampler is a motorized needle and a tray that withdraws and injects a certain amount of a prepared standard or sample into the LC machine.
The pump does just that...pumps the mobile phase with or without the sample/standard through the system
The column is a tube filled with different compounds that slow down the progress of the compounds in the standard or sample. Think of it as a tube filled with marbles. If you pour water through it, the water will eventually drain to the bottom. If you pour something more viscous, like oil, it will take a little longer because the substance is thicker than the water. If you try to pour something really viscous, like honey, it will take a lot longer because the substance is thicker than the oil. Molecules work the same way...some molecules are more attracted to the solid phase (the compound bonded into the inside of the column) than others. The ones that are less attracted to the column solid phase will work their way through the column faster than the compounds that are more attracted to the solid phase.
The UV Spectrometer reads when the compound makes it through the column. It detects the presence of the specific molecule by hitting it with a specific wavelength of light. The light interacts with the molecule, and the detector in the Spectrometer records this interaction.
The signal from the detector is then analyzed and sent to a recording software or to an integrator that prints out a chart.
The signals are represented as peaks. The location of the peak is the retention time of the specific molecule, and shows WHAT the molecule is. The area under the peak shows HOW MUCH of that molecule there is in the sample/standard.
The column we got is not separating the compounds we are looking at correctly, so we are waiting for another column that will (hopefully) separate them well enough to get some useable data so we can start analyzing the various polymers I have been making for the last 3 months
Last edited:
cbr_rachie
Club Member
okay i have no clue what that is lol!! HPLC....ummmm layman's terms please!
i work at ITT Water Equipment Technologies.....we work with filtration systems and reverse osmosis to make the dead sea drinkable lol....but i'm a temp here doing admin asst work so i'm bored
Rach
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Captain Slow
See post #4
cbr_rachie
Club Member
See post #4
geesh you post fast! yeh, well that sounds too complicated for me to handle, i'll stick to my office work for now!
you MUST be bored, you're all over on the forums posting!!!
Rach
cbr_rachie
Club Member
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Captain Slow
I'm an internet whore...I post all the time whether I'm bored or busy as fuck :lol:
And if you're THAT bored...this forum DOES have an "after hours" section, and I'd bet that your cell phone has a camera on it
And if you're THAT bored...this forum DOES have an "after hours" section, and I'd bet that your cell phone has a camera on it
91Bird305
Club Member
ohhhhhhmygosh, it's my favorite person in the world
pretty good. Check out the myspace site and look what I did. lol btw: cool pictures.
igotaneed4speed
Club Member
I stired up all kinds of shit apparently with the guys in emissions last night... Came intoday and got "talked to" about my informative e-mails...
cbr_rachie
Club Member
don't worry, i haven't forgotten.....i just wanted to see how fast you'd jump on this topic
how are ya? by the way, i LOVE ur trailblazer SS, sexy as hell.....and a 14.7 for a stock tb ss isn't anything to sneeze at.... i'd rock it